Table. 1-1 Antibody information.
Target | Molecular mass (kDa) | Isotype | Application | Company & Clone# |
|---|---|---|---|---|
CD73 | 73 | Mouse IgG | WB(1:3000) | Abcam/([1D7]) |
IHC(1:400) | ||||
β-actin | 37 | Mouse IgG | WB (1:3000) | CST/(8H10D10) |
Notch1 | 120 | Rabbit IgG | WB (1:3000) | CST/(C37C7) |
IHC(1:200) |
Tables 1–2 | Primers and used Small interference RNAs in this study. | ||
|---|---|---|---|
Target | 5′——3′ | ||
siRNA | Notch1-1 | 5-CACCAGUUUGAAUGGUCAATT-3 | |
Notch1-2 | 5-GUGUCUGAGGCCAGCAAGATT-3 | ||
CD73-1 | 5- TCTCAATCATGCCGCTTTA-3 | ||
CD73-2 | 5- GCCACTAGCATCTCAAATA-3 | ||
qRT-PCR | Notch1 | Forward | CGGGTCCACCAGTTTGAATG |
Reverse | GTTGTATTGGTTCGGCACCAT | ||
CD73 | Forward | GGTGGCTTT TAGGAT GGCAAG | |
Reverse | ACTGGAACGGTGAAGGTGACAG | ||
β-actin | Forward | GGTGGCTTTTAGGATGGCAAG | |
Reverse | ACTGGAACGGTGAAGGTGACAG | ||
ChIP | CD73 NC | Forward | AAAGCAAAGAAGGGCAGCAT |
Reverse | TGGGTTGAGAACGCACGA | ||
CD73 pro | Forward | GGCAATATGGGTGATGGGTATGTG | |
Reverse | CTTCTGGGACACCCTCTAATGTCATG | ||
