Fig. 2: NLRP3 deletion does not block HMGB1 release and ductular cell proliferation in the autophagy-deficient liver. | Cell Death Discovery

Fig. 2: NLRP3 deletion does not block HMGB1 release and ductular cell proliferation in the autophagy-deficient liver.

From: NRF2 transcriptionally regulates Caspase-11 expression to activate HMGB1 release by Autophagy-deficient hepatocytes

Fig. 2

A Representative gross morphology of livers of 9-week-old mice of the indicated genotypes. B, C The LW/BW ratio and the serum ALT levels were determined for 9-week-old mice. D Liver lysates from 9-week-old mice were analyzed by immunoblotting. E Liver sections were stained for HMGB1. Arrows indicate hepatocytes without nuclear HMGB1. Bar graph shows the percentage of hepatocytes with nuclear HMGB1. F Serum HMGB1 levels in 9-week-old mice (n = 3–4 mice/group). G Liver sections were subjected to H&E staining (×200) and immunostaining for CK19. H Liver sections were subjected to Sirius Red staining (×200) and immunostaining for F4/80 (×200). Data are expressed as the mean ± SEM. *P < 0.05, ***P < 0.01, n.s: not significant (n = 3).

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