Fig. 3: Cleavage of Gasdermin D in autophagy-deficient liver is inhibited by deletion of Caspase-11, but not Nlrp3. | Cell Death Discovery

Fig. 3: Cleavage of Gasdermin D in autophagy-deficient liver is inhibited by deletion of Caspase-11, but not Nlrp3.

From: NRF2 transcriptionally regulates Caspase-11 expression to activate HMGB1 release by Autophagy-deficient hepatocytes

Fig. 3

A, C Liver lysates from 9-week-old mice of the indicated genotypes were analyzed for proteolytic cleavage activity for Ac-LLSD-AFC. B, D Liver lysates from 9-week-old mice of the indicated genotypes were analyzed for the cleavage activity for Ac-YVAD-AFC. E, F Liver lysates from 9-week-old mice of the indicated genotypes were analyzed by immunoblotting. The GASDERMIN D (GSDMD) fragment, indicated by the arrow, was detected using an antibody specific to the N-terminal fragment. Bar graph shows the relative intensity of caspase-11 and cleaved GSDMD. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, n.s: not significant (n = 3).

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