Fig. 3: PEC exerts an anti-tumor effect by targeting TOP2A. | Cell Death Discovery

Fig. 3: PEC exerts an anti-tumor effect by targeting TOP2A.

From: Pectolinarigenin inhibits bladder urothelial carcinoma cell proliferation by regulating DNA damage/autophagy pathways

Fig. 3: PEC exerts an anti-tumor effect by targeting TOP2A.

A 3D structure of PEC. B Molecular docking of PEC and TOP2A or DNA-bound TOP2A by using AutoDockTools. C Western blots showing TOP2A protein level in BLCA cells incubated with indicated concentrations of PEC for 24 h. D, E The protein and transcript levels of TOP2A in different BLCA cell lines (5637, SCaBER, T24, UMUC3, J82). F Top: 5637 cell lysates were exposed for 30 min to DMSO or 100 μM PEC and subjected to CETSA. Bottom: the intensity of the TOP2A bands reflects binding affinity of PEC to TOP2A in 5637 cells. G MTT assay shows that knockdown TOP2A could reserve the inhibitory effect of PEC treatment for 48 h in 5637 cells. H Cell cycle analysis of 5637 and SCaBER cells transfected with siTOP2A or NC for 48 h, followed by treatment with or without PEC (10 μM in 5637, 20 μM in SCaBER) for 24 h. I Western blots showing related proteins in BLCA cells after knocking down TOP2A or exposing to PEC (10 μM in 5637, 20 μM in SCaBER) or combination.

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