Fig. 3: Gal-9-induced AML cell death does not rely on caspase-dependent apoptosis. | Cell Death Discovery

Fig. 3: Gal-9-induced AML cell death does not rely on caspase-dependent apoptosis.

From: Galectin-9 has non-apoptotic cytotoxic activity toward acute myeloid leukemia independent of cytarabine resistance

Fig. 3

PS-exposure detected by flow cytometry in Gal-9-treated (300 nM, 16 h). A THP-1 cells, CD34+ patient-derived AML cells in B liquid, or C MS5 co-cultures. D Quantification of PS exposure after blockade of pan-caspases with Z-VAD-FMK in Gal-9-treated THP-1 and HL-60 cells, using staurosporine (STS) as a positive control (n = 3). E As in D but determined on patient-derived CD34+ and CD34- cells. F Western blot of full-length caspase-3 (35 kDa) and cleaved caspase-3 (17 kDa) in HL-60 cells treated with Gal-9 (150, 300 nM, 16 h) or STS (25, 50 ng/ml, 16 h). Blockade of Gal-9-mediated PS-exposure with α-lactose (40 mM) in (G) a panel of AML cell lines (n = 5), H CD34+/CD34− patient-derived AML cells in liquid culture, or I in co-culture with MS5.

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