Fig. 3: The effect of Tim-3 on the expression of inflammatory factors.
From: Tim-3 protects against cisplatin nephrotoxicity by inhibiting NF-κB-mediated inflammation
A–C C57/BL6 wild-type and Tim-3 knockout mice were stimulated with cisplatin (30 mg/kg) for 72 h. IL-1β, TNF-α, and IL-10 mRNA levels were determined using quantitative RT-PCR (n = 3–5). D–F BUMPT cells were treated with 40 μM cisplatin with and without soluble Tim-3 recombinant protein for 24 h. IL-1β, TNF-α, and IL-10 mRNA levels were determined using quantitative RT-PCR (n = 3). Data were shown as means ± SD (n = 2–9 per group). Statistical significance was determined using two-way ANOVA followed by Tukey’s post hoc test. The experiments were repeated at least three times.
