Fig. 5: The effect of NF-κB inhibitors and sTim-3 on kidney tubular epithelial cells’ apoptosis. | Cell Death Discovery

Fig. 5: The effect of NF-κB inhibitors and sTim-3 on kidney tubular epithelial cells’ apoptosis.

From: Tim-3 protects against cisplatin nephrotoxicity by inhibiting NF-κB-mediated inflammation

Fig. 5: The effect of NF-κB inhibitors and sTim-3 on kidney tubular epithelial cells’ apoptosis.The alternative text for this image may have been generated using AI.

After pre-treatment with PDTC or TPCA1 for 1 h, BUMPT cells were stimulated by 40 μM cisplatin with and without sTim-3 for 24 h. A Hoechst 33342 staining of BUMPT cells in different groups. B The expression of P65, phosphor-p65, and cleaved caspase 3 were determined by western blot. GAPDH was used as the internal control. C The apoptotic rate was calculated as the percentage of Hoechst-stained cells over the total number of cells in the field. D, E The densities of western blot bands were quantified using ImageJ software. F Western blot analysis of the levels of cleaved caspase 3. GAPDH was used as the internal control. G The densities of western blot bands were quantified using ImageJ software. Data represent mean ± SD (n = 4 per group). Statistical significance was determined using two-way ANOVA (C) or one-way ANOVA (D, E, G) followed by Tukey’s post hoc test. The experiments were repeated at least three times.

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