Fig. 1: SCP2 expression is upregulated in human OA cartilage with ferroptosis.
From: SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis
A Transcription levels of 66 ferroptosis-related differentially expressed genes in human OA cartilage (n = 10 per group) compared with those in normal cartilage (n = 8 per group). B The differentially expressed proteins in the synovial fluid from rabbit joints were analyzed by volcano plots between injured cartilage (n = 3 per group) and normal cartilage (n = 3 per group). C Venn diagram showed the overlapped DEGs between transcriptomics and proteomics. D Positive cell proportions (%) of immunohistochemistry staining and iron staining in human OA (n = 5 per group) and normal (n = 3 per group) cartilage. E MDA contents of human primary chondrocytes from OA (n = 5 per group) and normal (n = 3 per group) cartilage. F Flow cytometry analysis of lipid peroxides level of human primary chondrocytes from OA (n = 5 per group) and normal (n = 3 per group) cartilage. G Cartilage sections were stained with DAB-enhanced Prussian blue to detect iron in human OA (n = 5 per group) and normal (n = 3 per group) cartilage. Scale bar, 200 μm (top), 20 μm (bottom). H Immunohistochemistry staining of COL2A1, MMP13, GPX4, and SCP2 in human OA (n = 5 per group) and normal (n = 3 per group) cartilage. Scale bar, 200 μm (top), 20 μm (bottom). Data are expressed as means + SD. Unpaired two-tailed t tests, *P < 0.05.
