Fig. 3: SCP2 mediates the membrane damage of mitochondria and lysosomes.
From: SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis
A Mitochondrial membrane potential of rat chondrocytes treated with RSL3 (0.25 μM), 4OHT (1 μM), or ScpI2 (5 μM) detected by JC-1 staining. Scale bar, 100 μm. B The mean intensity ratio of red fluorescence to green fluorescence in JC-1 staining (n = 3 per group). C Western blot of cytochrome C and VDAC in mitochondria after RSL3, 4OHT, or ScpI2 treatment (n = 3 per group). D Western blot of cytochrome C and GAPDH in cytoplasm after RSL3, 4OHT, or ScpI2 treatment (n = 3 per group). E ATP levels of rat chondrocytes after RSL3, 4OHT, or ScpI2 treatment (n = 3 per group). F Percentage of LDH release (%) of rat chondrocytes after RSL3, 4OHT, or ScpI2 treatment (n = 3 per group). G Lysosomal membrane permeabilization of rat chondrocytes treated with RSL3 (0.25 μM), 4OHT (1 μM), or ScpI2 (5 μM) detected by acridine orange staining. Scale bar, 50 μm. H The mean intensity ratio of red fluorescence to green fluorescence in acridine orange staining (n = 3 per group). I Lysosomes staining in rat chondrocytes treated with RSL3 (0.25 μM), 4OHT (1 μM), or ScpI2 (5 μM) detected by lyso-tracker red. Scale bar, 100 μm. J The mean fluorescence intensity in lyso-tracker red staining (n = 3 per group). K Trypan blue staining of rat chondrocytes under RSL3 (0.25 μM), 4OHT (1 μM), or ScpI2 (5 μM) treatment. Scale bar, 50 μm. L Positive cell proportions (%) in trypan blue staining (n = 3 per group). Data are expressed as means + SD. Unpaired two-tailed t tests, *P < 0.05.
