Fig. 4: SCP2 transports cytoplasmic LPO to mitochondria.
From: SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis
A The merged images of BODIPY 665/676, CellLight-mito-GFP, and Hoechst 33258 staining in rat chondrocytes treated with RSL3 (0.25 μM), 4OHT (1 μM), or ScpI2 (5 μM) were shown in the upper part. The merged images of immunofluorescence SCP2, CellLight-mito-GFP, and Hoechst 33258 staining in rat chondrocytes were shown in the lower part. Scale bar, 50 μm. B The relative colocalization coefficients of mitochondria and LPO with mito-GFP and BODIPY staining (n = 6 per group). C The relative colocalization coefficients of mitochondria and SCP2 with mito-GFP and immunofluorescence staining (n = 6 per group). D Western blot of SCP2 and VDAC in mitochondria after treatment of RSL3 (0.25 μM), 4OHT (1 μM), or ScpI2 (5 μM) (n = 3 per group). E SCP2 relative protein level in mitochondria after indicated treatment (n = 3 per group). F The mito-LPO was stained with Mito-PeDPP, and mito-ROS was stained with MitoSox in rat chondrocytes after RSL3 (0.25 μM), 4OHT (1 μM), MitoQ (1 μM), or ScpI2 (5 μM) treatment. Scale bar, 100 μm. G The mean fluorescence intensity for Mito-LPO staining (compared to blank group of Mito-LPO, *P < 0.05), and Mito-ROS staining (compared to blank group of Mito-ROS, #P < 0.05), n = 6 per group. H The binding rates of SCP2 to 15(S)-HpETE or GSH were detected in phosphate buffered saline by LC-MS/MS (n = 3 per group). Unpaired two-tailed t tests, *P < 0.05.
