Fig. 2: Schematic of different strategies to vascularization of brain organoid.
From: Human 3D brain organoids: steering the demolecularization of brain and neurological diseases
A Microinjection of brain organoids in mice brain led to engraftment and invasion by host vasculature. B Co-culture of PSCs with endothelial cells and C genetic engineering of inducible transcription factor ETV2 in hESCs led to vascular system development in brain organoids. D EBs early fed with endothelial factors such as VEGF and later with WNT7 display vascularization along with neuronal differentiation. E Different fusion approaches led to brain organoid vascularization include fusion of (i) neural and mesodermal spheroid (ii) brain EB sandwiched with vascular EB in a Matrigel droplet (iii) brain EB and dissociated vascular organoids.
