Fig. 4: TRPA1 activation induces mitochondrial Ca2+ overload in primary cultures of mCRC cells. | Cell Death Discovery

Fig. 4: TRPA1 activation induces mitochondrial Ca2+ overload in primary cultures of mCRC cells.

From: Transient receptor potential ankyrin 1 (TRPA1) mediates reactive oxygen species-induced Ca2+ entry, mitochondrial dysfunction, and caspase-3/7 activation in primary cultures of metastatic colorectal carcinoma cells

Fig. 4: TRPA1 activation induces mitochondrial Ca2+ overload in primary cultures of mCRC cells.

A Mitochondrial Ca2+ signals induced by AITC (30 µM) in mCRC cells maintained under control conditions (Ctrl), pretreated with HC-030031 (30 µM, 30 min) or with the highly specific inhibitor of the mitochondrial Ca2+ uniporter, Ru360 (5 µM, 30 min), or transfected with the selective siTRPA1. B Mean ± SE of peak mitochondrial Ca2+ signal evoked by AITC in control (Ctrl) mCRC cells and in mCRC cells transfected with siTRPA1 or pretreated with HC-030031 or Ru360. One-way ANOVA followed by the post hoc Dunnett’s test. ***p < 0.001. The numbers placed above the scattered dots represent the number of responding cells out of the total cell number. N = 4 for each experimental condition. C Mitochondrial Ca2+ signals induced by H2O2 (50 µM) in mCRC cells maintained under control conditions (Ctrl), pretreated with HC-030031 (30 µM, 30 min) or with the highly specific inhibitor of the mitochondrial Ca2+ uniporter, Ru360 (5 µM, 30 min), or transfected with the selective siTRPA1. D Mean ± SE of peak mitochondrial Ca2+ signal evoked by H2O2 in control (Ctrl) mCRC cells and in mCRC cells transfected with siTRPA1 or pretreated with HC-030031 or Ru360. One-way ANOVA followed by the post hoc Dunnett’s test. ***p < 0.001. The numbers placed above the scattered dots represent the number of responding cells out of the total cell number. N = 4 for each experimental condition. E Mitochondrial Ca2+ signals induced by 4-HNE (30 µM) in mCRC cells maintained under control conditions (Ctrl), pretreated with HC-030031 (30 µM, 30 min) or with the highly specific inhibitor of the mitochondrial Ca2+ uniporter, Ru360 (5 µM, 30 min), or transfected with the selective siTRPA1. F Mean ± SE of peak mitochondrial Ca2+ signal evoked by 4-HNE in control (Ctrl) mCRC cells and in mCRC cells transfected with siTRPA1 or pretreated with HC-030031 or Ru360. One-way ANOVA followed by the post hoc Dunnett’s test. ***p < 0.001. The numbers placed above the scattered dots represent the number of responding cells out of the total cell number. N = 4 for each experimental condition.

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