Fig. 2: Characterization of transformed fibroblast cell lines capable and incapable of reprogramming to iPSC-like cells.

a Representative brightfield and GFP images from reprogrammed transformed MEFs for the indicated cell lines. Scale bar = 100 µm. b Chart showing the number of Oct4-GFP+ colonies from the indicated transformed MEF lines. Each MEF line was at least 1 month old. Reprogramming was performed between three and five independent biological replicates (See Supplementary Table 1). Circles represent biological replicates; the red line indicates the mean and the error bars indicate the standard error of the mean (SEM). c Heatmap from the RNA-seq data showing the expression of a representative set of genes specific to somatic fibroblastic cells, pluripotent stem cells, or from the MET (mesenchymal-epithelial transition). NTC=normalized tag count. d Correlation heatmap (R²) of the original MEF samples and the reprogrammed GFP+ cells and ESCs. e PCA scatter plot showing the first two dimensions of the RNA-seq data from the original wildtype or transformed MEF lines (red) and the GFP+ cell lines (blue) and ESCs (green) maintained in serum+LIF or 2i+LIF.