Fig. 6: FOXM1 directly promotes the transcription of SET7.
From: FOXM1 maintains fatty acid homoeostasis through the SET7-H3K4me1-FASN axis
A A ChIP-PCR assay was performed in GSC 4121 and GSC 3691 cells. B Left, heatmap of gene peaks in GSC 4121 cells. Right, FASN peaks in GSC 4121 cells. C A Co-IP assay was performed in GSC 4121 cells using anti-H3 and anti-H4 antibodies, and histone methylation was detected using LC‒MS analysis. Y-axes: -log10 p-values. Activating and repressive indicate different functions in epigenetic regulation. D H3K4me1 was detected using immunoblotting in GSC 4121 and GSC 3691 cells (uncropped western blots are details in Original Data File). E Graphical schematic of the screening strategy and the resulting Venn diagram. F, G The mRNA and protein levels of SET7 were measured in cells with the indicated modifications (uncropped western blots are details in Original Data File). Error bar represent three independent experiments, ***p < 0.001. ***p < 0.001. H Graphical schematic of different primers. Relative mRNA levels were determined using ChIP-PCR (ENCODE SCREEN: P1: EH38E2329942, P2: EH38E2329943, P3: EH38E2329943). I The luciferase reporter plasmid was transfected into GSC 4121 and GSC 3691 cells. Relative luciferase activity was determined by calculating the Rluc/Luc ratio. Error bar represent three independent experiments, ***p < 0.001. ***p < 0.001. J A SET-MUT reporter plasmid lacking the binding site P1 was constructed. SET-WT and SET-MUT were transfected into GSCs, and relative luciferase activity was measured. Error bar represent three independent experiments, ***p < 0.001. ***p < 0.001.
