Fig. 7: The FOXM1-SET7-H3K4me1 axis maintains FASN expression.

A ChIP-PCR was performed using an anti-H3K4me1 antibody to detect the FASN promoter. B FOXM1 K.O. cells were transfected with a SET7 overexpression plasmid, and FOXM1 OV cells were treated with the SET7-specific inhibitor PFI-2. The levels of FOXM1, SET7, H3K4me1 and FASN were determined using immunoblotting (uncropped western blots are details in Original Data File). C, D The relative FA content was measured in cells with the indicated modifications. Error bar represent three independent experiments, ***p < 0.001. E, F BSP was performed to determine the FASN promoter methylation level. A representative image and the statistical analysis results are presented. Error bar represent three independent experiments, ***p < 0.001. G, H ChIP was performed to detect the involvement of DNMT1/DNMT3B. Error bar represent three independent experiments, ***p < 0.001. I The levels of FOXM1, SET7, H3K4me1 and FASN in GBM samples were determined using immunoblotting (uncropped western blots are details in Original Data File). J The relative FASN level was determined using qPCR, BSP was then performed to determine the FASN promoter methylation rate, and the proportion of hypermethylation/hypomethylation was calculated. ***p < 0.001.