Fig. 2: POLI facilitates the repair of DNA DSBs in esophageal squamous cell carcinoma cells via homologous recombination. | Cell Death Discovery

Fig. 2: POLI facilitates the repair of DNA DSBs in esophageal squamous cell carcinoma cells via homologous recombination.

From: Polymerase iota (POLI) confers radioresistance of esophageal squamous cell carcinoma by regulating RAD51 stability and facilitating homologous recombination

Fig. 2: POLI facilitates the repair of DNA DSBs in esophageal squamous cell carcinoma cells via homologous recombination.The alternative text for this image may have been generated using AI.

A DNA double-strand breaks (DSBs) damage to ESCC cells treated with IR (4 Gy) was detected by using the neutral single-cell gel electrophoresis assay (comet assay). Scale bar, 20 μm. B Immunofluorescent staining of γ-H2AX foci was adopted at different time points after 4 Gy IR and a measured number of foci per cell. Scale bar, 5 μm. C, D CRISPR/Cas9-induced oligodeoxynucleotide (ODN) detecting system was applied to monitor the efficiencies of NHEJ and HR. E Immunofluorescent staining of RAD51 foci was performed at several time points after 4 Gy IR and a measured number of foci per cell. Scale bar, 10μm. F Western blotting analysis of POLI, RAD51, and Ku70/80 expression at different times points following 4 Gy IR treatment. Data was shown as mean ± SEM. P value was measured by Student’s t test; ***P < 0.00, **P < 0.01, *P < 0.05.

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