Fig. 1: FGF19 induces cell cycle arrest of chondrocytes at the G2/M Phase.

A CCK-8 assay showing the changes in cell viability of chondrocytes induced by 200 ng/ml FGF19 for 72 h with KLB (200 ng/ml). KLB, β Klotho. B EDU staining indicating DNA synthesis of chondrocytes induced by 200 ng/ml FGF19 for 72 h with KLB (200 ng/ml). C Quantitative analysis of EdU-positive cells in (B). D Cell cycle changes based on flow cytometry showing cell cycle G2/M phase arrest of chondrocytes induced by 200 ng/ml FGF19 for 72 h in the presence of KLB (200 ng/ml). E Quantitative analysis of cell populations in (D). F Pheatmap showing the related gene changes of cell cycle arrest in chondrocytes based on RNA sequencing. Rows represent genes, and columns represent different groups. The red indicates the upregulation of gene expression, while the green indicates the downregulation of genes. The datasets were presented in FPKM and were clustered using the online R package. Three independent cell isolates yielded three pairs of lysate samples (n = 3). Each pair sprang from identical maternal cells. The data in A, C and E were presented as the means ± SD, and the significant data presented in A, C and E were based on two-tailed Student’s t-tests. Experiments presented in A, B, D and F were based on at least three independent repeats (n ≥ 3).