Fig. 2: FGF19 mediates cell cycle arrest of chondrocytes at the G2 phase by down-regulation of cdk1 and cylinb1 and up-regulation of chk1 and gadd45a.
From: FGF19 induces the cell cycle arrest at G2-phase in chondrocytes
A Western blot showing the expression change of cdk1, cylinb1, chk1 and gadd45a in chondrocytes stimulated with 0, 50, 100 and 200 ng/ml FGF19 for 72 h in the presence of the corresponding concentration of KLB (0, 50, 100 and 200 ng/ml). B Quantitative analysis presenting the protein expression of cdk1, cylinb1, chk1 and gadd45a in chondrocytes in (A). C Representative immunofluorescent images showing the reduced expression of cdk1 in chondrocytes induced by 200 ng/ml FGF19 for 72 h with KLB (200 ng/ml). Cytoskeleton, green; Cdk1, red; nucleus, blue. D Linear fluorescent quantification presenting the distribution of cdk1 in chondrocytes in (C). E Representative immunofluorescent images showing the reduced nuclear expression of cylinb1 in chondrocytes induced by 200 ng/ml FGF19 for 72 h with KLB (200 ng/ml) by CLSM. Cytoskeleton, green; Cylinb1, red; nucleus, blue. F Quantification of the linear fluorescent protein of cylinb1 showing the cylinb1 intracellular distribution in chondrocytes in (E). The data in B, D and F are presented as the means ± SD. The significant analysis in B was based on two-tailed Student’s t-tests. All results in A, C and E were obtained from three independent experiments (n = 3).
