Fig. 3: FGF19 induces cell cycle arrest at G2 phase through FGFR4.
From: FGF19 induces the cell cycle arrest at G2-phase in chondrocytes
A Representative q-PCR results showed that FGF19 (200 ng/ml) and KLB stimulate chondrocytes could increase mRNA expression of FGFR4. B Representative western blots confirming the change in protein expression of FGFR4 in chondrocytes induced by 200 ng/ml FGF19 with KLB. C Representative immunofluorescent pictures presenting chondrocytes treated with 200 ng/ml FGF19 and KLB with higher FGFR4 expression. Cytoskeleton, green; FGFR4, red; nucleus, blue. D Flow cytometry analysis demonstrated that 5 μM BLU9931 attenuated 200 ng/ml FGF19-induced cell cycle G2 phase arrest in the presence of KLB. E Quantitative analysis of cell populations in (D). F Representative western blots showed that FGF19 (200 ng/ml) combined with KLB stimulation of chondrocytes causes changes in the expression of FGFR4, cdk1, cylinb1, chk1 and gadd45a in the presence or absence of BLU9931 (5 μM). G Representative immunofluorescent pictures presenting the increase of cdk1 in chondrocytes after pretreatment with 5 μM BLU9931 for 2 h in the presence of FGF19 (200 ng/ml) and KLB for 72 h. Cytoskeleton, green; Cdk1, red; nucleus, blue. H Quantification of the linear fluorescent intensity of cdk1 in chondrocytes shown in (G). I Representative immunofluorescent pictures presenting chondrocytes with higher cylinb1 expression pretreated with 5 μM BLU9931 for 2 h in the presence of FGF19 and KLB for 72 h. Cytoskeleton, green; Cylinb1, red; nucleus, blue. J Quantification of the linear fluorescent intensity of cylinb1 in chondrocytes shown in (I). The data in A, E, H, and J were presented as the means ± SD, and the significant data presented in A and E were based on two-tailed Student’s t-tests. Experiments presented in B–D, F, G and I were based on at least three independent repeats (n ≥ 3).
