Fig. 5: Blocking Akt2 attenuates diabetes-induced RPE cell migration. | Cell Death Discovery

Fig. 5: Blocking Akt2 attenuates diabetes-induced RPE cell migration.

From: Aberrant Akt2 signaling in the RPE may contribute to retinal fibrosis process in diabetic retinopathy

Fig. 5

Human fetal RPE (fRPE) cells were transfected with and without non-relevant siRNA (siCtrl) or anti-Akt2 siRNA (siAkt2) for 24 h in 5 mM and 25 mM d-Glucose culture medium, and a wound healing experiment was performed. A Representative phase-contrast microscope images showing the area covered by the human fRPE cells at 0 and 24 h after wounding. B High glucose (25 mM) increased cell migration compared to the low glucose condition (5 mM), and blocking Akt2 signaling using Akt2 siRNA significantly inhibited diabetes-induced human fRPE cell migration compared to the control group (siCtrl). Cell migration was determined by the percentage of cells within the scratched area using ImageJ™ software. Data are collected from four replicates from each group and shown as mean ± SD. **p < 0.01 and ****p < 0.0001. Statistical test used in this study is One-way ANOVA followed by Tukey’s post-hoc test. Scale bar = 100 μm.

Back to article page