Fig. 2: Inhibition of EV-production interferes with primary cSCC tumor growth in vivo. | Cell Death Discovery

Fig. 2: Inhibition of EV-production interferes with primary cSCC tumor growth in vivo.

From: Cutaneous squamous cell carcinoma-derived extracellular vesicles exert an oncogenic role by activating cancer-associated fibroblasts

Fig. 2: Inhibition of EV-production interferes with primary cSCC tumor growth in vivo.

A The efficiency of shRNA-mediated knockdown of RAB27A in UT-SCC-7 cells was determined by qRT-PCR. ****P < 0.0001, Student’s t test, n = 4. B Production of EVs by control or RAB27 shRNA-transduced UT-SCC-7 cells was analyzed by NTA. ***P < 0.001, Student’s t test, n = 3. C Primary tumor growth was monitored after the subcutaneous injection of control (shCtrl) and RAB27A-knockdown (shRAB27A) UT-SCC-7 cells into immunocompromised mice. *P < 0.05, ****P < 0.0001. Two-way ANOVA. Six immunocompromised mice were used for xenograft models. D Representative images of tumors formed on mice at week 4, 5, and 6. E Weight of tumors formed by control and RAB27A-knockdown UT-SCC-7 cell lines was measured after the termination of the experiment at week 6. *P < 0.05, Wilcoxon matched-pairs sighed rank test, 6 xenografts per group. Data are presented as mean ± SEM.

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