Fig. 4: cSCC cells stimulate the transformation of fibroblasts to cancer-associated fibroblasts via soluble mediators. | Cell Death Discovery

Fig. 4: cSCC cells stimulate the transformation of fibroblasts to cancer-associated fibroblasts via soluble mediators.

From: Cutaneous squamous cell carcinoma-derived extracellular vesicles exert an oncogenic role by activating cancer-associated fibroblasts

Fig. 4: cSCC cells stimulate the transformation of fibroblasts to cancer-associated fibroblasts via soluble mediators.

A Schematic representation of the co-culture assay of human dermal fibroblasts (HDFs) and NHEK or cSCC cells. Created using BioRender (BioRender.com). B Cancer-associated fibroblast (CAF)-related genes were detected in HDFs co-cultured with NHEK or cSCC cells by qRT-PCR. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. not significant, one-way ANOVA, n = 4. C, D Expression of α-SMA in mouse stromal cells in shRAB27A vs. shCtrl cSCC xenografts was measured by RNAseq (log2 -normalized read counts, C) and qRT-PCR analysis (D). ***P < 0.001, Student’s t-test, 6 xenografts per group. Data are presented as mean ± SEM.

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