Fig. 5: cSCC cell-derived EVs are taken up by fibroblasts and drive the onset of cancer-associated fibroblast phenotypes. | Cell Death Discovery

Fig. 5: cSCC cell-derived EVs are taken up by fibroblasts and drive the onset of cancer-associated fibroblast phenotypes.

From: Cutaneous squamous cell carcinoma-derived extracellular vesicles exert an oncogenic role by activating cancer-associated fibroblasts

Fig. 5: cSCC cell-derived EVs are taken up by fibroblasts and drive the onset of cancer-associated fibroblast phenotypes.

A Schematic representation of the treatment of HDFs with NHEK- or cSCC cell-derived EVs (corresponding to 20 µg of protein). Created using BioRender (BioRender.com). B Uptake of cSCC cell-derived EVs by HDFs was visualized by labeling the EV-cargo (RNA) with SYTO RNASelect green fluorophore. NC: HDFs cultured without EVs added; EVs: HDFs incubated with UT-SCC-7-derived, fluorescently labeled EVs. F-actin was stained with phalloidin, DNA was stained with DAPI. Scale bar = 100 µm. C The expression of CAF-marker genes was analyzed by qRT-PCR in HDFs treated with EVs (corresponding to 20 µg of protein) produced by NHEK or cSCC cell lines. *P < 0.05, **P < 0.01, ****P < 0.0001, n.s. not significant, one-way ANOVA, n = 4. Data are presented as mean ± SEM.

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