Fig. 1: Screening and characteristics of m6A-related circRNF13 in cervical cancer. | Cell Death Discovery

Fig. 1: Screening and characteristics of m6A-related circRNF13 in cervical cancer.

From: circRNF13, a novel N6-methyladenosine-modified circular RNA, enhances radioresistance in cervical cancer by increasing CXCL1 mRNA stability

Fig. 1: Screening and characteristics of m6A-related circRNF13 in cervical cancer.The alternative text for this image may have been generated using AI.

A, B After the gene sequencing of the radiosensitive and radioresistant tissues of cervical cancer, the heat map and volcano plot (Fold Change > 2, P < 0.05) of the differential circRNAs were analyzed. C Me-RIP screened the methylation modification of candidate circRNAs. D hsa_circ_0001346 is formed by the circularization of RNF13 gene, which was verified by Sanger sequencing. E cDNA and genomic gDNA reverse transcribed from circRNF13 were subjected to PCR amplification in SiHa and HeLa cells. F Differences between circRNF13 and linear RNA upon RNase R treatment in SiHa and HeLa cells. G Nucleocytoplasmic separation assay to characterize the distribution of circRNF13 in SiHa and HeLa cells. H The distribution of circRNF13 was examined by FISH assay in SiHa and HeLa. (***P < 0.001, **P < 0.01, *P < 0.05).

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