Fig. 1: NPCs in culture readily release EVs. | Cell Death Discovery

Fig. 1: NPCs in culture readily release EVs.

From: Extracellular vesicles from neural progenitor cells promote functional recovery after stroke in mice with pharmacological inhibition of neurogenesis

Fig. 1

A Schematic representation of EV collection from primary NPCs in culture. B Neurospheres grown in vitro for 4 days. Bar equals 50 µm. C Expression of neuronal progenitor markers nestin (green) and doublecortin (red). Bar equals 10 µm. D NPC’s growing in monolayers in vitro. Bar equals 20 µm. E OGD-induced luciferase reporter gene expression at different time points following reoxygenation. F Expression of HIF-1 protein at 2 and 4 h after OGD in NPCs. G Expression of VEGF mRNA at 2 and 10 h after OGD in NPCs. H Transmission electron microscopy micrographs of EVs isolated from NPCs cultures for 24 h. Vesicles were visualized by negative staining with uranyl formate on copper/carbon-coated grids. Bar equals 200 nm. I Size distribution of particles in suspensions by nanoparticle tracking analysis of EVs isolated under normoxia for 48 h and after a 2-h OGD stimulus and 42-h recovery.

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