Fig. 2: NPC-EV protect cortical neurons against ischemic death. | Cell Death Discovery

Fig. 2: NPC-EV protect cortical neurons against ischemic death.

From: Extracellular vesicles from neural progenitor cells promote functional recovery after stroke in mice with pharmacological inhibition of neurogenesis

Fig. 2

A Schematic representation of the experimental design to expose cortical neurons to OGD-induced injury and administration of NPC-EVs. B Representative images of cortical neurons under control conditions (right) and following 1 h OGD (left). CytoCalcein stain labels alive neurons (green), while dead cells are marked by the nuclear staining of 7-AAD (red) for necrotic cells or apopxin (magenta) for apoptotic cells. Bar equals 100 µm. Assessment of neuronal viability by MTT reduction of cultures exposed to 1 h OGD (C), 100 µM NMDA (D), 10 µM HNE (E), or 10 nM ST (F), supplemented with Nx-EV or OGD-EV. Graphs show the mean ± S.D. with individual experiments plotted to show the number of independent experiments and data distribution per condition. ANOVA followed by Tukey’s post hoc.

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