Fig. 7: BEL inhibited ISO-induced cardiac hypertrophy via BRD4/NOX4. | Cell Death Discovery

Fig. 7: BEL inhibited ISO-induced cardiac hypertrophy via BRD4/NOX4.

From: Bellidifolin ameliorates isoprenaline-induced cardiac hypertrophy by the Nox4/ROS signalling pathway through inhibiting BRD4

Fig. 7

A–E H9C2 cells were transfected with Nox4 lentiviral and infected with adenovirus overexpressing BRD4 with or without BEL and ISO treatment. The protein levels of ANP, BNP, BRD4, and Nox4 were determined by Western blot. GAPDH was used as a loading control (n = 3). F–J H9C2 cells were transfected with si-Nox4 and infected with adenovirus overexpressing BRD4. Finally, cells were treated either with or without BEL and ISO. The protein levels of ANP, BNP, BRD4 and Nox4 were determined by Western blot. GAPDH was used as a loading control (n = 3). Data are shown as the mean ± SEM. Significance: *p < 0.05 vs CON group; **p < 0.01 vs CON group; #p < 0.05 vs ISO group; ##p < 0.01 vs ISO group; $p < 0.05 vs ISO + BEL group ; $$p < 0.01 vs ISO + BEL group; Δp < 0.05 vs ISO + BEL + BRD4 group; ΔΔp < 0.01 vs ISO + BEL + BRD4 group ; ns no significant difference.

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