Fig. 1: Pyroptosis and apoptosis both occur in the skeletal muscle of the mouse CI model. | Cell Death Discovery

Fig. 1: Pyroptosis and apoptosis both occur in the skeletal muscle of the mouse CI model.

From: Delayed step-by-step decompression with DSF alleviates skeletal muscle crush injury by inhibiting NLRP3/CASP-1/GSDMD pathway

Fig. 1: Pyroptosis and apoptosis both occur in the skeletal muscle of the mouse CI model.

a–c qPCR analyses the mRNA level of pyroptosis-associated molecular Casp-1, GSDMD, IL-18 in the skeletal muscle under 1.5 kg pressure for the different indicated crush times (0.5, 2, 12, and 16 h). d WB analyses NLRP3, Casp-1, cleaved-Casp-1, GSDMD, N-GSDMD, IL-1β, cleaved- IL-1β, and IL-18 protein expression in the skeletal muscle under different crush times. e IHC staining analyses NLRP3, cleaved-Casp-1, N-GSDMD and cleaved-IL-1β protein expression in the skeletal muscle (original magnification: 400×; scale bar: 100 μm). f, g Quantification of the integrated optical density (IOD) of the image in (e). h, i qPCR analyses the mRNA level of apoptosis-associated molecular Bax and Casp-3 in the skeletal muscle. j WB analyses Bax, Casp-3, cleaved-Casp-3, PARP, and cleaved-PARP protein expression in the skeletal muscle. k IHC staining analyses Bax, cleaved-Casp-3, cleaved-PARP protein expression in the skeletal muscle (original magnification: 400×; scale bar: 100 μm). l, m Quantification of the integrated optical density (IOD) of the image in (k). One-way ANOVA was used in (a–c, f–i, l, m) (n = 6).

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