Fig. 7: Delayed step-by-step decompression and/or pyroptotic inhibitor therapy alleviates skeletal muscle and kidney injury of CS-AKI mice induced by CI.

a Schematic drawing of the experimental design of the treatment of CI mice. CI represents that the mouse was crushed under 1.5 kg for 12 h. CI/SR represents that the mouse was crushed under 1.5 kg for 12 h, then maintained squeezed under 0.5 kg pressure for an additional 2 h. NC + DSF represents that mouse was intraperitoneal injection DSF for 4 h. CI + DSF represents that mouse was crushed under 1.5 kg for 10 h, administration of DSF, and maintained at 1.5 kg for an additional 2 h before direct decompression and reperfusion for 2 h. CI/SR + DSF represents that mouse was crushed under 1.5 kg for 10 h, administration of DSF, and maintained at 1.5 kg for an additional 2 h before maintained squeezed under 0.5 kg pressure for an additional 2 h. b–f CK, Mb, LDH, BUN, and Scr concentration in serum. g HE staining analyses skeletal muscle and kidney pathological changes under different crush conditions for 12 h (NC, CI, CI/SR, NC + DSF, CI + DSF, CI/SR + DSF). IHC detected NGAL expression in the kidney of different groups (NC, CI, CI/SR, NC + DSF, CI + DSF, CI/SR + DSF) (original magnification: 400×; scale bar: 100 μm). One-way ANOVA was used in (b–f) (n = 6).