Fig. 3: FOXD1 directly combined with the p21 promoter regions and downregulates its expression. | Cell Death Discovery

Fig. 3: FOXD1 directly combined with the p21 promoter regions and downregulates its expression.

From: miR-30e-5p-mediated FOXD1 promotes cell proliferation by blocking cellular senescence and apoptosis through p21/CDK2/Rb signaling in head and neck carcinoma

Fig. 3: FOXD1 directly combined with the p21 promoter regions and downregulates its expression.The alternative text for this image may have been generated using AI.

A KEGG enrichment analyses between sh-FOXD1#NC and sh-FOXD1#1 SCC25 cells indicated enrichment in tumor progression. B Genes involved in the cellular senescence pathway are shown in the heatmap. C Volcano plot analysis indicated that p21 was notably upregulated in sh-FOXD1#1 SCC25 cells. D qRT-PCR and E western blot analyses clarified that p21 expression was remarkably increased in FOXD1 knockdown cells but significantly decreased in FOXD1-overexpressing cells. F Three regions (S1–S3) of the p21 promoter containing FOXD1-binding sites were predicted by JASPAR. G Luciferase assays confirmed that the relative luciferase activity was increased when S3 region was mutated. H ChIP-qPCR assay revealed the enrichment of FOXD1 on the p21 promoter (region 2) was significantly downregulated in FOXD1-overexpressed SCC25 cells (IgG was used as a control). *p < 0.05, **p < 0.01, and ***p < 0.001. FOXD1, forkhead box D1; KEGG, Kyoto Encyclopedia of Genes and Genomes; SEM standard error of the mean, ns negative significance, qRT-PCR quantitative real-time polymerase chain reaction, ChIP-qPCR chromatin immunoprecipitation-quantitative polymerase chain reaction.

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