Fig. 1: PME-1 expression sensitizes GBM cells to oxidative stress. | Cell Death Discovery

Fig. 1: PME-1 expression sensitizes GBM cells to oxidative stress.

From: PME-1 sensitizes glioblastoma cells to oxidative stress-induced cell death by attenuating PP2A-B55α-mediated inactivation of MAPKAPK2-RIPK1 signaling

Fig. 1

A Endogenous PME-1 levels in U87MG and U251MG cells were determined and quantified using Western blotting (N = 3, two-tailed t-test); a representative blot is shown. B Sensitivity of U87MG and U251MG cells to 16 h t-BHP treatment (different concentrations) was determined using MTT (N = 3, two-way ANOVA). C Validation of stable PME-1-GFP overexpression in U87MG cells using Western blotting (left). Quantification below the blots refers to ‘total’ PME-1 levels ( = endogenous PME-1 + PME-1-GFP). The sensitivity of U87MG PME-1-GFP cells to 16 h treatment with indicated concentrations of t-BHP was determined using MTT (N = 4, two-way ANOVA) (right). D Validation of stable PME-1 knockdown in U251MG cells using Western blotting (left). The sensitivity of U251MG shPME-1 cells to 16 h treatment with indicated concentrations of t-BHP was determined using MTT (N = 5, two-way ANOVA) (right).

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