Fig. 7: STAT3 and STAT5 have a conserved function in primary myeloid progenitor cells and in vivo.

A, B The bone marrow-derived myeloid progenitor cells were isolated from 6-week-old mice. The primary cells were pretreated with STAT3-IN (A) or STAT5-IN (B) according to the indicated concentrations, and induced by 2 ng/mL G-CSF and GM-CSF for 24 h. The cells were collected to detect the indicated protein markers with western blotting. C The primary cells were pretreated with 5 μM STAT3-IN or STAT5-IN, followed by differentiation medium treatment only (Control, CTL) or were induced by 10 ng/mL G-CSF or/and GM-CSF for 5 days. The cells were stained with Wright-Giemsa (left). The bar stands for 50 μm. The cell number of the indicated types was counted (right). The details of statistical analyses on cell numbers in indicated types were shown in Fig. S7. D 10 mg/kg STAT3-IN or STAT5-IN were injected into the 6-week-old male mice intraperitoneally every 2 days. After 7 days, peripheral blood and bone marrow cells were separated, and the levels of CD11b, Ly6G and F4/80 were detected by flow cytometry. The data are the mean ± SD, *P < 0.05.