Fig. 6: Knocking down RAB14 inhibited autophagy in BLCA cells. | Cell Death Discovery

Fig. 6: Knocking down RAB14 inhibited autophagy in BLCA cells.

From: RAB14 promotes epithelial-mesenchymal transition in bladder cancer through autophagy‑dependent AKT signaling pathway

Fig. 6

A Immunofluorescent staining showing the number of LC3 punctas in the T24-mRFP-GFP-LC3 KD cells and their control cells. Cells were stained by indirect immunofluorescence using anti-LC3 antibody, and the number of LC3 puncta per cell was quantified. Scale bars = 100 μm. B Representative TEM ( × 20,000) images of T24 cells and quantification of autophagic vacuoles (autophagosomes and autolysosomes) in subsets of 10 randomly-selected cells of each type. Red arrows, autophagic vacuoles. C Western blotting assay showed that the expression of LC3B and Beclin1 decreased and P62 showed the opposite change with the knocking down of RAB14. *P < 0.05, **P < 0.001.

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