Fig. 7: The activation of autophagy reversed RAB14-promoted EMT, migration and invasion in bladder cancer cells. | Cell Death Discovery

Fig. 7: The activation of autophagy reversed RAB14-promoted EMT, migration and invasion in bladder cancer cells.

From: RAB14 promotes epithelial-mesenchymal transition in bladder cancer through autophagy‑dependent AKT signaling pathway

Fig. 7

A Immunofluorescence staining of E-cadherin. T24 cells were treated with rapamycin (RAPA), which is a specific inhibitor of mTOR protein, to study the effect of autophagy. Immunofluorescent staining showed that shRAB14 + RAPA group showed similar levels of E-cadherin as shNC group, but it showed E-cadherin was down-regulated compared with shRAB14 group. B Immunofluorescence staining of Vimentin. Immunofluorescent staining showed that shRAB14 + RAPA group showed similar levels of vimentin as shNC group, but vimentin was significantly upregulated compared with shRAB14 group. C Representative TEM ( × 20,000) images of T24 cells and quantification of autophagic vacuoles (autophagosomes and autolysosomes) in subsets of 10 randomly-selected cells of each type. D Western blotting assay of E-cadherin, N-cadherin and Vimentin. E Cell migration and invasion were ananlyzed. *P < 0.05, **P < 0.001.

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