Fig. 2: Circ235 promotes Bca cell migration, proliferation, and aerobic glycolysis. | Cell Death Discovery

Fig. 2: Circ235 promotes Bca cell migration, proliferation, and aerobic glycolysis.

From: Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p

Fig. 2

A, B T24 and UMUC3 cells underwent transfection with siRNAs targeting circ235 or were infected with circ235-overexpressing plasmids using lentiviral tools, and circ235’s relative expression was quantified using qRT-PCR and normalized using β-actin. C, D The significant reduction in Bca cells’ proliferative capacity was observed following knockdown of circ235. E Representative images of Bca cells underwent transfection with si-circ235 in the colony formation assay. F Statistical diagrams of Bca cells’ clonogenic abilities with circ235 knockdown. G Representative images of Bca cells underwent transfection with si-circ235 in the wound-healing assay. H Statistical diagrams of Bca cells’ migration abilities with circ235 knockdown in a wound-healing assay. I Representative images of Bca cells underwent transfection with si-circ235 in the transwell assay. J Statistical diagrams of T24 and UMUC3 cells’ migration abilities with circ235 knockdown in the transwell assay. K The relative glucose consumption of Bca cells with circ235 knockdown using the glucose assay kit. L The relative lactate production in Bca cells with circ235 knockdown using the lactate assay kit. M The extracellular acidification rate (ECAR) was quantitated using the Seahorse XFe96 Extracellular Flux Analyzer. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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