Fig. 1: Identification and characteristics of an I-125-related circRNA in HCC. | Cell Death Discovery

Fig. 1: Identification and characteristics of an I-125-related circRNA in HCC.

From: Characterization of circSEC11A as a novel regulator of Iodine-125 radioactive seed-induced anticancer effects in hepatocellular carcinoma via targeting ZHX2/GADD34 axis

Fig. 1

A Heatmap showing the up- and down-regulation circRNAs after treated with I-125 in HepG2 cells. B qRT-PCR for detecting the original expression level of circSEC11A in HepG2, QSG-7701, Huh7, and SMMC7721 cells. C qRT-PCR for detecting the expression level of circSEC11A in Huh7 and HepG2 cells. D Schematic diagram of chromosomal location and formation of circSEC11A. E Agarose gel electrophoresis was performed to detect the amplification of circSEC11A and GAPDH using convergent and divergent primers in cDNA and gDNA. F Relative RNA level of circSEC11A, linear SEC11A, and GAPDH treated with RNase R. G Relative RNA level of circSEC11A, linear SEC11A, and GAPDH treated with Act-D at the indicated time. H Flurorescence in situ hybridization (FISH) was used to detect the localization of circSEC11A (red) in Huh7 and HepG2 cells. Cell nuclei were stained with DAPI (blue). The data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page