Fig. 3: CircSEC11A promotes the I-125-induced anticancer effect in HCC via functions as a miR-3529-3p sponge. | Cell Death Discovery

Fig. 3: CircSEC11A promotes the I-125-induced anticancer effect in HCC via functions as a miR-3529-3p sponge.

From: Characterization of circSEC11A as a novel regulator of Iodine-125 radioactive seed-induced anticancer effects in hepatocellular carcinoma via targeting ZHX2/GADD34 axis

Fig. 3

A The heatmap of the differentially expressed miRNAs in HepG2 cells treated with I-125. B Veen diagram to identify miRNA predicted to bind with circSEC11A by circBANK (www.circbank.cn), CSCD (gb.whu.edu.cn/CSCD), and miRNA-seq (Control vs I-125). C qRT-PCR to detect the expression level of miR-3529-3p after circSCE11A was down- or up-regulated in Huh7 or HepG2 cells. D Relative luciferase activity were detected in cells co-transfected with circSEC11A-WT/circSEC11A-Mut and miR-NC/miR-3529-3p mimics in Huh7 and HepG2 cells. E RIP was performed with anti-AGO2 or IgG antibodies and the enrichment of circSEC11A and miR-3529-3p were detected via qRT-PCR in Huh7 cells. F RNA pull-down assay was performed using a circSEC11A probe and the enrichment of miR-3529-3p was detected via qRT-PCR in Huh7 cells. G The location of circSEC11A and miR-3529-3p in Huh7 cells were detected with FISH assay. H–K CCK-8, flow cytometry, and wound healing assay were performed to detect cell proliferation, apoptosis, and metastasis in HepG2 and Huh7 cells treated with I-125 and co-transfected with sh-NC/sh-circSEC11A and miR-3529-3p NC/inhibitor or vector/circSEC11A and miR-3529-3p NC/mimics. The data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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