Fig. 7: GADD34 is a transcriptional target of ZHX2. | Cell Death Discovery

Fig. 7: GADD34 is a transcriptional target of ZHX2.

From: Characterization of circSEC11A as a novel regulator of Iodine-125 radioactive seed-induced anticancer effects in hepatocellular carcinoma via targeting ZHX2/GADD34 axis

Fig. 7: GADD34 is a transcriptional target of ZHX2.

A, B Based on the results of RNA-seq performed with SMMC7721 cells transfected with control-RNAi or ZHX2-RNAi, the volcano map and heatmap show the differentially expressed mRNAs. C, D The KEGG pathway enrichment and GO annotation of the differentially expressed mRNAs in RNA-seq. E, F The KEGG pathway enrichment and GO annotation of the differentially expressed mRNAs in ChIP-seq performed with SMMC7721 cells transfected with control-RNAi or ZHX2-RNAi. G qRNA for detecting the GADD34 expression level in HepG2 and SMMC7721 cells after transfected with pcZHX2 or ZHX2-RNAi. H The dual luciferase reporter assay for detecting GADD34 promoter activity in HepG2 and SMMC7721 cells after transfected with pcZHX2 or ZHX2-RNAi. I The ChIP assay to detect ZHX2 occupancy on the promoter of GADD34 in HepG2 and SMMC7721 cells. The quantification of qRT-PCR (left) and semiquantitative images (right) are shown. The data are presented as the mean ± SD. *P < 0.05, **P < 0.01.

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