Fig. 2: Characterization of PANPs.
From: Nanoparticles carrying paclitaxel and anti-miR-221 for breast cancer therapy triggered by ultrasound
A, B Representative images of PANPs by TEM (transmission electron micrograph) (A) and PEM (projection electron microscopy) (B). C Size of PANPs in DMEM at 37 °C. D Zeta-potential of PANPs at 37 °C measured using a Zeta dynamic light scattering analyzer. E The anti-miR-221 was stained using Cy5 after fluorescence microscopy of the observed images, and its content was detected by fluorescence spectrophotometry (green for the shell and red for anti-miR-221). F The Cy5 fluorescence intensity in E shows a dose-dependent manner. G Representative images of PFP vaporization in PANPs in DMEM by ultrasonic stimulation with intensities of 0, 0.6, 0.8, and 1.0 W/cm2. H High-performance liquid chromatography (HPLC) analysis of the PTX release from PANPs in DMEM upon ultrasound sonication showing an intensity-dependent manner. I Ultrasound images of PANPs in Matrigel before and after sonication with different intensities of 0.6, 0.8, and 1.0 W/cm2 for different times from 0 to 20 s. The left figure is an ultrasonography image, and the right is a conventional two-dimensional image. J Quantitative analysis of echo signal intensities in I. Data are presented as the mean ± SEM (n = 3). **P < 0.01.
