Fig. 2: Haspin-KO increases the ratio of ACD and causes defective development capacity.

A Schematic illustration of the steps for the induction of asymmetric stem cell division (ACD). B Representative images of WT, Haspin-KO, and rescued mESCs cultured with N2B27 medium and stained with Nanog and Tubulin antibodies. Scale bar: 10 μm. ACD: asymmetric stem cell division, SCD: symmetric stem cell division. C The percentages of ACD and SCD were defined by Nanog fluorescence intensity in WT, Haspin-KO, and rescued mESCs. n = 150 from three independent experiments. D Colony morphology and AP staining of WT and Haspin-KO and rescued mESCs. Scale bar: 100 μm. E Schematic illustration of the steps for embryoid body (EB) formation. Phase-contrast microscopy images of EBs obtained from mESCs on Day 3 and Day 6. Scale bar: 50 μm. F The area statistics of EB. n = 20 for Day 3 and Day 6. G Schematic illustration of the teratoma formation and tissue analysis. Teratomas formed from nude mice subcutaneously injected with WT or Haspin-KO mESCs at 4 weeks. H The statistical analysis of teratoma weights (n = 4). The data in (C), (F), and (H) are represented as the mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001.