Fig. 1: Alcohol induces ferroptosis both in vitro and in vivo. | Cell Death Discovery

Fig. 1: Alcohol induces ferroptosis both in vitro and in vivo.

From: Ferroptosis contributes to ethanol-induced hepatic cell death via labile iron accumulation and GPx4 inactivation

Fig. 1

A Cell viability analysis of ethanol-exposed AML12 cells pretreated with relative cell death inhibitors. AMl12 cells were pretreated with 25 μM Nec-1, 20 μM Z-VAD-FMK, 1 μM Ferr-1, 1 μM Lip-1, or 10 μM DFO for 3 h, and then cells were exposed to 400 mM ethanol for 24 h in the presence of relative cell death inhibitors. Cell viability was measured using CCK8 assay. B, C Liver H&E staining (B) and Oil-Red O staining (C) from mice fed with 8-week ethanol plus one binge (E8w+1B) or pair-fed mice. D Serum non-esterified fatty acids (NEFA) levels in E8w+1B mice and pair-fed mice. E Figure illustration of the pathway involved in fatty acid uptake and phospholipid hydroperoxide synthesis. F Heatmap showing protein levels of CD36, LPCAT3, ACSL4, and POR in the liver of E8w+1B mice and pair-fed mice. The expression profiles were retrieved from our TMT proteomics data. G Immunoblotting analysis of the expressions of CD36, LPCAT3, ACSL4, and POR in the liver of E8w+1B mice and pair-fed mice. H–J Liver concentrations of ferrous irons (H), ferric irons (I), and total irons (J) in E8w+1B mice and pair-fed mice. K The ratios of ferrous irons to ferric irons in the liver of E8w+1B mice and pair-fed mice. L–N The levels of lipid peroxidation products 15(S)-HETE (L), 15,16-DiHODE (M), and MDA (N) in the liver of E8w+1B mice and pair-fed mice. O Representative transmission electron microscopy (TEM) images of the liver from E8w+1B mice and pair-fed mice. n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001 as indicated.

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