Fig. 8: AGO2 participates in the positive regulation of miR-214. | Cell Death Discovery

Fig. 8: AGO2 participates in the positive regulation of miR-214.

From: Ferroptosis contributes to ethanol-induced hepatic cell death via labile iron accumulation and GPx4 inactivation

Fig. 8

A Schematic illustration of RIP analysis of the interaction between miR-214 and AGO1 or AGO2 proteins in the cytoplasm and nucleus of mouse primary hepatocytes. B RIP analysis of miR-214 enrichment in the cytoplasm and nucleus using AGO1 and AGO2 antibody. Immunoblotting analysis of the expressions of ACSL4 and PRKAA2 protein in primary hepatocytes co-transfected with miR-214 mimics (20 nM) and/or AGO1 siRNAs (C) or AGO2 siRNAs (D). Data are expressed as means ± SD from at least three independent experiments. ns not significant; *P < 0.05, **P < 0.01, and ***P < 0.001.

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