Fig. 7: SCARB1 regulates HAAO and CYP1B1 levels via the transcription factor KLF9. | Cell Death Discovery

Fig. 7: SCARB1 regulates HAAO and CYP1B1 levels via the transcription factor KLF9.

From: SCARB1 in extracellular vesicles promotes NPC metastasis by co-regulating M1 and M2 macrophage function

Fig. 7

A Base peak plot of the IP mass spectra of SCARB1. B IP experiments to validate the binding of SCARB1 to KLF9. C Prediction of possible KLF9-target genes. D Luciferase reporter assay of HAAO and CYP1B1 transcriptional activity. E Cellular immunofluorescence co-localization of SCARB1 with KLF9. F ChIP assay for validation of possible KLF9-target genes. G HAAO and CYP1B1 protein levels in the cells. H ROS flow statistics of M1 macrophages (two-way ANOVA). I Statistics of CCK8 assay in M1 macrophages (Two-way ANOVA). J Images of M2 macrophages engulfing latex beads. K Staining diagram of M2 macrophages with Wright-Giemsa. The arrow represents M2 macrophages after phagocytosis of CNE2 cells. L, M Phagocytosis statistics of M2 macrophages (*p < 0.05, **P < 0.01, ***p < 0.001, Student’s t-test).

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