Fig. 6: Survivin overexpressed in 5-Fu resistant CRC cells. | Cell Death Discovery

Fig. 6: Survivin overexpressed in 5-Fu resistant CRC cells.

From: Targeting survivin with Tanshinone IIA inhibits tumor growth and overcomes chemoresistance in colorectal cancer

Fig. 6: Survivin overexpressed in 5-Fu resistant CRC cells.

A the IC50 of HT29/HT29R and HCT116/HCT116R cells with 5-Fu treatment. MTS and soft agar assays were used to detect cell viability (B) and colony formation (C) of HT29/HT29R and HCT116/HCT116R cells. D, E The in vivo tumorigenesis of HCT116 and HCT116R cells. D tumor volume; E tumor mass and weight. F, G The in vivo tumorigenesis of HT29 and HT29R cells. F tumor volume; G tumor mass and weight. H IB assay was used to detect survivin expression in HCT116/HCT116R and HT29/HT29R cells. I Knockdown of survivin in HCT116R and HT29R cells was measured by IB assay. Survivin knockdown HCT116R and HT29R cells were treated with 5-Fu (30 µM) or DMSO control and subjected to MTS (J) and soft agar (K) analysis. (L) IB assay of apoptosis in survivin knockdown HT29R with 5-Fu treatment. *P < 0.05, ***P < 0.001.

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