Fig. 2: Dosage effect of the NAF-144–67 peptide on MCF-10A, MDA-MB-231, and MDA-MB-157 cells.

A–E Time course line graph analyses (left) and bar graphs showing statistical analyses of cell death at 24 h (right) induced in MCF-10A, MDA-MB-231 and MDA-MB-157, by the application of 0 (A), 5 (B), 10 (C), 15 (D), and 30 (E) μM of NAF-144–67. All experiments were repeated at least 3 times with 3 different technical repeats per cell line. Statistical significance was determined using one-way ANOVA. NAF-1 nutrient autophagy factor 1, PI propidium iodide, AU arbitrary units.