Fig. 8: A hypothetical model for the effects of the NAF-144–67 peptide on MCF-10A, MDA-MB-231, and MDA-MB-157 cells in the presence or absence of cycloheximide. | Cell Death Discovery

Fig. 8: A hypothetical model for the effects of the NAF-144–67 peptide on MCF-10A, MDA-MB-231, and MDA-MB-157 cells in the presence or absence of cycloheximide.

From: A combination of a cell penetrating peptide and a protein translation inhibitor kills metastatic breast cancer cells

Fig. 8

The barrier of control MCF-10A cells to the peptide is shown to be unaffected by cycloheximide (CHX) and these cells are resistant to the peptide in the presence or absence of CHX. In contrast, MDA-MB-231 cells cannot block the peptide from entering and are susceptible to the peptide in the presence or absence of CHX. In the absence of CHX, MDA-MB-157 cells can block the peptide from entering and are therefore more resistant to it than MDA-MB-231 cells. However, in the presence of CHX, MDA-MB-157 cells are unable to block the peptide from entering and are sensitive to it. The model supports the use of a cell penetrating peptide-antibiotic/chemotherapy combination treatment against primary and metastatic cancer cells. CHX cycloheximide, NAF-1 nutrient autophagy factor 1.

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