Fig. 7: IL-6 secreted by cancer cells plays a critical role in M2-like macrophage polarization. | Cell Death Discovery

Fig. 7: IL-6 secreted by cancer cells plays a critical role in M2-like macrophage polarization.

From: Tumor suppressor p53 mediates interleukin-6 expression to enable cancer cell evasion of genotoxic stress

Fig. 7

A THP-1 monocytes were differentiated into M0 macrophages, co-cultured with control or IL-6-silenced A549 cells with and without SA/Dox treatment to induce macrophage polarization, and co-cultured with parental A549 cells to stimulate cancer cell migration and invasion as depicted. B Flow cytometric analysis of cell surface expression of the M1- (CD80) and M2-associated (CD206) macrophage markers in macrophages co-cultured with control (scramble) or IL-6-silenced (shIL-6) A549 cells with and without 20 µM SA or 0.1 µM Dox treatment for 24 h. Cells were double-stained with FITC-labeled anti-CD80 and APC-labeled anti-CD206 antibodies. C, D The (C) migratory and (D) invasive abilities of A549 cells co-cultured with macrophages stimulated with RPMI, control (scramble), or IL-6-silenced (shIL-6-1 and shIL-6-2) A549 cells with and without 20 µM SA or 0.1 µM Dox treatment for 24 h were measured with transwell assays. Scale bar: 100 µm. Error bars represent mean ± SD, n = 3. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ns not significant.

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