Fig. 5: Autophagy inhibition enhances necroptosis.

A The mRNA levels of autophagy markers (LC3B, ATG16L1, and ULK1) and necroptosis markers (RIPK1, RIPK3, and MLKL) in ATP7B R778L mutant HepG2 cell line with or without spautin-1 treatment. B The protein levels of autophagy markers (ULK1, P-ULK1, and Beclin1) and necroptosis markers (RIPK3 and p-MLKL) in ATP7B R778L mutant HepG2 cell line with or without spautin-1 treatment. *P < 0.05, **P < 0.01. Spa, spautin-1.