Fig. 8: Knockdown of ULK1 and ATG16L1 enhances necroptosis. | Cell Death Discovery

Fig. 8: Knockdown of ULK1 and ATG16L1 enhances necroptosis.

From: ATP7B R778L mutant hepatocytes resist copper toxicity by activating autophagy and inhibiting necroptosis

Fig. 8: Knockdown of ULK1 and ATG16L1 enhances necroptosis.

A The mRNA levels of ULK1 in ATP7B R778L-mutant HepG2 cell line treated with ULK inhibitor-ULK101. B The mRNA levels of necroptosis markers (RIPK3 and MLKL) in ATP7B R778L-mutant HepG2 cell line treated with ULK inhibitor-ULK101. C The protein levels of p-ULK1 and necroptosis markers (p-RIPK3 and p-MLKL) in ATP7B R778L-mutant HepG2 cell line treated with ULK101. D The mRNA levels of ATG16L1 in ATP7B R778L-mutant HepG2 cell line transfected with lentivirus-enveloped shATG16L1. E The mRNA levels of necroptosis markers (RIPK3 and MLKL) in ATP7B R778L-mutant HepG2 cell line transfected with lentivirus-enveloped shATG16L1. F The protein levels of p-ATG16L1 and necroptosis markers (p-RIPK3 and p-MLKL) in ATP7B R778L-mutant HepG2 cell line transfected with lentivirus-enveloped shATG16L1.

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