Fig. 2: Cdk12 limits axonal b-actin patches.
From: Cdk12 maintains the integrity of adult axons by suppressing actin remodeling
A A representative b-actin fluorescence intensity plot of small focal b-actin patches in wild type in wing sensory neurons at 21 days. B Aged wild-type axons at 35 days also display small patches of actin. C Cdk12−/− axons display large axon patches that are intensely bright at 21 days. D By 35 days Cdk12−/− axons the b-actin fluorescence intensity plot displays multiple bright axon b-actin patches up to 50 μm away from cell body. E Cdk12−/− axons displayed a significantly greater area under the curve of fluorescent b-actin intensity plots. F Quantification shows that loss of Cdk12 is associated with increased b-actin patch formation. G The total fluorescence intensity of Cdk12−/− axons was greater than control at both 21 days and 35 days. Fluorescence was normalized to td-Tomato expressed in the same cell to label membranes. Data was analyzed by two-way ANOVA and significant differences annotated as p < 0.05* & p < 0.01** between genotypes. Graphs are expressed as Mean ± SEM and N = ≥ 8 wings for each group. Scale bar = 10 µm.
